研討會主題：

Employing new approaches toinvestigate human and mouse group 2 innate lymphoid cell responses

采用新方法研究人和小鼠二型固有淋巴細胞（ILC2s）免疫應答

注冊網址：https://labroots.com/register/webcast-collection/97

主講人：

Elia Tait Wojno

AssistantProfessor, Cornell University College of Veterinary Medicine

會議時間：

日期：2015年6月16日

時間：8:00AM Pacific time, 11:00AM Eastern Time

北京時間：2015年6月15日下午17：00

會議內容：

二型固有淋巴細胞（ILC2s）是近期被報道有助于2型炎癥、組織修復和調節代謝平衡的先天免疫細胞。ILC2s 在各種生物作用中扮演著重要的角色，由于這類細胞比較稀少，使得對其表型和功能分析存在一定技術難度。這次網絡研討會將會討論用于檢測小鼠模型和人體組織動態細胞群的前沿技術，集中評估了前列腺素D2受體，CRTH2（Th2細胞表達的化學引誘物同源分子）的作用，前列腺素D2受體和CRTH2在ILC2免疫應答里用強化選擇的技術方法來分析ILC2的表型和功能。這種技術包括多色流式細胞術，細胞分選處理，單細胞RNA染色（PrimeFlow™ RNA assay）和ILC2體外功能分析。另外，我們會介紹新推出的產品PrimeFlow™ RNA assay，它能在單細胞水平同時檢測細胞中的RNA和蛋白質。這次會議我們將會為研究ILC2的研究人員提供ILC領域的相關信息。

Abstract

Date: June 16,2015

Time: 8:00AMPacific time, 11:00AM Eastern Time

Group 2 innatelymphoid cells (ILC2s) are recently described innate immune cells thatcontribute to type 2 inflammation, tissue repair, and metabolic homeostasis inmultiple tissues. While ILC2s play key roles in various biological processes,these cells are rare, making analysis of their phenotype and function atechnical challenge. This webinar will discuss cutting-edge techniques that canbe used to examine this dynamic cell population in murine models and in humantissues. A project focused on assessing the role of the prostaglandin D2receptor, CRTH2 (chemoattractant homologous molecule expressed by Th2 cells),in ILC2 responses will be used to highlight selected technical approaches andassays that can be used to analyze ILC2 phenotype and function. Techniques thatwill be highlighted include multi-color flow cytometry, cell sortingapproaches, single-cell RNA staining, and in vitro assays for ILC2 function. Inparticular, the PrimeFlow™ RNA assay, which enables simultaneous assessment ofprotein and RNA transcript expression on a single cell level, will bediscussed. The key concepts described during the session will provide relevantinformation for those new to the ILC field and for investigators who arealready experienced in the analysis of ILC responses.